Journal: International Journal of Molecular Sciences
Article Title: Different Metabolism and Toxicity of TRANS Fatty Acids, Elaidate and Vaccenate Compared to Cis-Oleate in HepG2 Cells
doi: 10.3390/ijms23137298
Figure Lengend Snippet: Viability of cell treated with fatty acids at various concentrations. Cells were treated with ( a ) BSA-conjugated palmitate, oleate, elaidate or vaccenate (100–800 µM) or with ( b ) palmitate (800 µM) and oleate, elaidate or vaccenate at a 4:1 or 2:1 molar ratio (200 or 400 µM) at 70–80% confluence for 24 h. Cell viability was assessed by the Colorimetric (MTT) Kit for Cell Survival and Proliferation (Millipore) and expressed as the percentage of the control. Data are shown as mean values ± S.D.; n = 18; statistically significant differences: * p < 0.05 vs. BSA treated control; ! p < 0.05 vs. 800 µM palmitate treated samples; # p < 0.05 vs. palmitate and oleate treated cells in case of combinational treatments at the corresponding ratio.
Article Snippet: Cell viability was assessed by using the Colorimetric (MTT) Kit for Cell Survival and Proliferation (Millipore, Burlington, MA, USA) according to the manufacturer’s instructions.
Techniques: Control